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GATE 2017-2018 Biotechnology Set 1

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1. In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by




2. The basis for blue-white screening with pUC vectors is




3. Idiotypic determinants of an antibody are associated with the




4. Identification of blood groups involves




5. B-lymphocytes originate from the bone marrow whereas T-lymphocytes originate from




6. A humanized antibody is one in which the




7. Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because




8. Nude mice refers to




9. Heat inactivation of serum is done to inactivate




10. A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with




11. A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?




12. Protein-DNA interactions in vivo can be studied by




13. The direction of shell coiling in the snail Limnaea peregra is a classic example of




14. During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield




15. Which one of the following is NOT a protoplast fusion inducing agent?




16. The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is




17. Identify the statement that is NOT applicable to an enzyme catalyzed reaction.




18. An example of a derived protein structure database is




19. An example of a program for constructing a phylogenetic tree is




20. Synteny refers to




21. While searching a database for similar sequences, E value does NOT depend on the




22. In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with




23. The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately




24. The helix content of a protein can be determined using




25. Which one of the following DNA sequences carries an invert repeat?




26. In zinc finger proteins, the amino acid residues that coordinate zinc are




27. Match the entries in Group I with those in Group II. Group I Group II P. MTT 1. Dihydrofolate reductase Q. Annexin V 2. Succinate dehydrogenase R. Methotrexate 3. Microtubules S. Taxol 4. Phosphatidylserine




28. In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 gl-1 (DCW), the specific growth rate (μ) is 0.4 h-1 and substrate uptake rate (v) is 16 gl-1h-1. The cell yield coefficient Yx/s will be




29. A single base pair of DNA weighs 1.1 * 10-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 μg of purified DNA?