R4RIN – MCQs, Mock Tests | Gate GATE 2017-2018 Biotechnology Set 1

In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by

1. acetylation
2. phosphorylatio
3. methylation
4. glycosylation

✅ Correct Answer: 3

The basis for blue-white screening with pUC vectors is

1. intraallelic complementation
2. intergenic complementation
3. intragenic suppression
4. extragenic suppression

✅ Correct Answer: 1

Idiotypic determinants of an antibody are associated with the

1. constant region of the heavy chains
2. constant region of the light chains
3. variable region
4. constant regions of light and heavy chains

✅ Correct Answer: 3

Identification of blood groups involves

1. precipitation
2. neutralization
3. opsonization
4. agglutination

✅ Correct Answer: 4

B-lymphocytes originate from the bone marrow whereas T-lymphocytes originate from

1. thymus
2. bone marrow
3. spleen
4. liver

✅ Correct Answer: 2

A humanized antibody is one in which the

1. heavy and light chains are from human
2. heavy chain is from human and light chain is from mouse
3. light chain is from human and heavy chain is from mouse
4. CDRs are from mouse, and the rest is from human

✅ Correct Answer: 4

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

1. it is an organic solvent
2. it easily penetrates cells
3. it protects cells by preventing crystallization of water
4. it is also utilized as a nutrient

✅ Correct Answer: 3

Nude mice refers to

1. mice without skin
2. mice without thymus
3. knockout mice
4. transgenic mice

✅ Correct Answer: 2

Heat inactivation of serum is done to inactivate

1. prions
2. mycoplasma
3. complement
4. pathogenic bacteria

✅ Correct Answer: 3

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with

1. Glycine
2. alanine
3. aspartate
4. threonine

✅ Correct Answer: 3

A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?

1. Ribosomal protein gene
2. Transfer RNA gene
3. DNA repair gene
4. Ribosomal RNA gene

✅ Correct Answer: 2

Protein-DNA interactions in vivo can be studied by

1. gel shift assay
2. Southern hybridization
3. chromatin immunoprecipitation assay
4. fluorescence in situ hybridization assay

✅ Correct Answer: 3

The direction of shell coiling in the snail Limnaea peregra is a classic example of

1. chromosomal inheritance
2. extra-chromosomal inheritance
3. chromosomal translocation
4. homologous recombination

✅ Correct Answer: 2

During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield

1. one molecule each of 3-phosphoglycerate and 2-phosphoglycolat
2. two molecules of 3-phosphoglycerate
3. two molecules of 2-phosphoglycolate
4. one molecule each of 3-phosphoglycerate and glyoxylate

✅ Correct Answer: 1

Which one of the following is NOT a protoplast fusion inducing agent?

1. Inactivated Sendai virus
2. Ca2+ at alkaline pH
3. Polyethylene glycol
4. Colchicine

✅ Correct Answer: 4

The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is

1. 1.66 * 104 IU
2. 60 IU
3. 6 * 107 IU
4. 106 IU

✅ Correct Answer: 3

Identify the statement that is NOT applicable to an enzyme catalyzed reaction.

1. Enzyme catalysis involves propinquity effects
2. The binding of substrate to the active site causes a strain in the substrate
3. Enzymes do not accelerate the rate of reverse reaction
4. Enzyme catalysis involves acid-base chemistry

✅ Correct Answer: 3

An example of a derived protein structure database is

1. Pfam
2. SCOP
3. GEO
4. Prosite

✅ Correct Answer: 2

An example of a program for constructing a phylogenetic tree is

1. phylip
2. phrap
3. prodom
4. PHDsec

✅ Correct Answer: 1

Synteny refers to

1. gene duplication from a common ancestor
2. a tree representation of related sequences
3. the extent of similarity between two sequences
4. local conservation of gene order

✅ Correct Answer: 4

While searching a database for similar sequences, E value does NOT depend on the

1. sequence length
2. number of sequences in the database
3. scoring system
4. probability from a normal distribution

✅ Correct Answer: 4

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with

1. ferrous ammonium sulfat
2. uranium acetate
3. sodium chloride
4. basic fuchsin

✅ Correct Answer: 2

The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately

1. 0.855
2. 5.55
3. 8.5
4. 55.5

✅ Correct Answer: 3

The helix content of a protein can be determined using

1. an infrared spectrometer
2. a fluorescence spectrometer
3. a circular dichroism spectrometer
4. a UV-Visible spectrophotometer

✅ Correct Answer: 3

Which one of the following DNA sequences carries an invert repeat?

1. ATGAGCCCCGAGTA TACTCGGGGCTCAT
2. ATGAGCCGGCTCTA TACTCGGCCGAGAT
3. ATGAGCCGAGCCTA ACTCGGCTCGGAT
4. ATGAGCCTATGGTA TACTCGGATACCAT

✅ Correct Answer: 2

In zinc finger proteins, the amino acid residues that coordinate zinc are

1. Cys and His
2. Asp and Glu
3. Arg and Lys
4. Asp and Arg

✅ Correct Answer: 1

Match the entries in Group I with those in Group II. Group I Group II P. MTT 1. Dihydrofolate reductase Q. Annexin V 2. Succinate dehydrogenase R. Methotrexate 3. Microtubules S. Taxol 4. Phosphatidylserine

1. P-3, Q-1, R-4, S-2
2. P-2, Q-4, R-1, S-3
3. P-2, Q-3, R-4, S-1
4. P-4, Q-2, R-1, S-3

✅ Correct Answer: 2

In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 gl-1 (DCW), the specific growth rate (Î¼) is 0.4 h-1 and substrate uptake rate (v) is 16 gl-1h-1. The cell yield coefficient Yx/s will be

1. 0.32
2. 0.64
3. 0.8
4. 0.50

✅ Correct Answer: 4

A single base pair of DNA weighs 1.1 * 10-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 Î¼g of purified DNA?

1. 0.30
2. 0.55
3. 0.25
4. 0.91

✅ Correct Answer: 2